Alhamfaib Ardananurdin, Eko Agus Subagio, Budi Utomo
Background: Acute spinal cord injury (ASCI) is one of the causes of morbidity that is severe enough to cause vascular damage. This vascular damage will cause necrosis or apoptosis. ACTH4-10Pro8-Gly9-Pro10 is one of the peptide groups that can affect peripheral nerve regeneration and rehabilitation.
Objective: This study was aimed to test the effect of ACTH4-10Pro8-Gly9-Pro10 on spinal cord’s IL-1, TNF-α and NF-κB expression in sprague-dawley rat on ASCI.
Methods: This research is a true experimental laboratory research with completely randomized design (factorial pattern). The subjects were Sprague Dawley rats with light and heavy compression of the spinal cord was performed respectively.
Results: : In rats with mild ASCI given ACTH4-10Pro8-Gly9-Pro10, IL-1, TNF-α, NF-kB expression results were 5.14 ± 1.95, 4 ± 1.73, 6.43 ± 1.61 and 10.71 ± 3.14, 6.29 ± 1.70, 11.14 ± 1.77 after 3 and 6 hours period of time respectively. In rats with severe ASCI given ACTH4-10Pro8-Gly9-Pro10 given ACTH4-10Pro8-Gly9-Pro10, IL-1, TNF-α, NF-kB results were 9.57 ± 1.81, 9.43 ± 1.81, 8, 71 ± 1.25 and 14.14 ± 2.11, 14.29 ± 2.28, 13.57 ± 1.81 after 3- and 6-hours period of time respectively. The administration of ACTH4-10Pro8-Gly9-Pro10 showed the results of IL-1, TNF-α, NF-kB expression were able to reduce IL-1, TNF-α and NF-kB expression when compared to NaCl 0.9% treatment group.
Conclusion: The administration of ACTH4-10Pro8-Gly9-Pro10 can reduce the expression of IL-1, TNF-α and NF-kB at 3 hours and 6 hours after mild and severe ASCI in Sprague Dawley rats. Future researches are recommended.
Keywords: ACTH4-10Pro8-Gly9-Pro10, Acute spinal cord injury, IL-1, NF-kB, TNF-α
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