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The effect of ACTH4-10Pro8-Gly9-Pro10 on spinal cord’s bax concentration in sprague-dawley rat on acute spinal cord injury

Mohamad Arif Fauzi , Eko Agus Subagio, Budi Utomo

Mohamad Arif Fauzi
Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Academic Medical Center Hospital. Email: docter_ariffauzi@yahoo.com

Eko Agus Subagio
Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Academic Medical Center Hospital

Budi Utomo
Department of Neurosurgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Academic Medical Center Hospital
Online First: December 01, 2019 | Cite this Article
Fauzi, M., Subagio, E., Utomo, B. 2019. The effect of ACTH4-10Pro8-Gly9-Pro10 on spinal cord’s bax concentration in sprague-dawley rat on acute spinal cord injury. Indonesian Journal of Neurosurgery 2(3). DOI:10.15562/ijn.v2i3.45


Introduction: Acute spinal cord injury (ASCI) triggers an inflammatory response that causes apoptosis. ACTH4-10Pro8-Gly9-Pro10 has a neuromodulator effect that can inhibit apoptosis. This effect is expected to help prevent deterioration in ASCI. This study aimed to explain the effect of giving ACTH4-10Pro8-Gly9-Pro10 to the value of Bax expression in the experimental animals with ASCI.

Methods: This study was a true experimental laboratory study with a complete randomized design factorial pattern. The subjects of the study were Sprague Dawley rats. Light and heavy compression of the spinal cord was performed on 12 subjects respectively. All subjects were then terminated and spinal cord tissue was taken for Bax analysis and histopathological cell apoptosis.

Results: In mice with mild ASCI given ACTH4-10Pro8-Gly9-Pro10, the results of Bax expression were 3.67 ± 2.08 and 8.67 ± 1.53 after 3 hours and 6 hours respectively, lower than the group with administration of 0.9% NaCl, which was equal to 11 ± 1 and 13.67 ± 0.58 (p < 0.05). In the heavy ASCI treatment group given ACTH4-10Pro8-Gly9-Pro10 the results of Bax expression were 9 ± 2 and 12.33 ± 2.08 after 3 hours and 6 hours respectively, lower than the group with 0.9% NaCl, that was equal to 18 ± 2.64 and 19 ± 2 (p < 0.05).

Conclusion: Giving ACTH4-10Pro8-Gly9-Pro10 in the first 3 hours yield greater decrease in Bax expression compared to the first 6 hours, despite insignificantly. Administration of ACTH4-10Pro8-Gly9-Pro10 can reduce Bax expression in ASCI. Future researches are recommended.

Keywords: acute spinal cord injury, ACTH4-10Pro8-Gly9-Pro10, apoptosis, bax

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